This research presented a novel as well as an easy method for a green synthesis of silver nanoparticles (AgNPs) using autoclave assisted gum extract of sesbania gum. Silver nanoparticles were formed due to the reduction of silver nitrate solution when mixed with the gum extract after autoclaving at 121°C and 15 psi. The water soluble components in the gum act as reductants and stabilizers. The generated nanoparticles were analyzed using uv-visible spectroscopy, transmission electron microscopy, x-ray diffraction, fourier transform-infrared spectroscopy. The antibacterial activity of the synthesized nanoparticles was tested on model gram-negative and gram-positive bacterial strains with the well-diffusion method. These nanoparticles exhibited considerable antibacterial activity on both the Gram classes of bacteria, implying their potential biomedical applications.
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